Journal: Perfusion

Article Title: Platelet count reduction during in vitro membrane oxygenation affects platelet activation, neutrophil extracellular trap formation and clot stability, but does not prevent clotting

doi: 10.1177/0267659121989231

Figure Lengend Snippet: Display of: (a) platelets (PLTs), (b) platelet activation (CD62+/CD61+), (c) neutrophils and (d) neutrophil extracellular trap formation (NET formation) over time. Baseline (BL). * p < 0.05. ** p < 0.01.*** p < 0.001.

Article Snippet: Briefly, 1:50 diluted whole blood samples from the loop were incubated with fluorescein isothiocyanate (FITC)-conjugated and phycoerythrin (PE)-conjugated CD61 and CD62P (MCA2263F, MCA2418PE, BioRad Inc., Feldkirchen, Germany) or their appropriate isotype controls (MCA928F, MCA 928PE, BioRad Inc., Feldkirchen, Germany) for 15 minutes at room temperature as described previously.

Techniques: Activation Assay

Journal: The Journal of Veterinary Medical Science

Article Title: Anti-Inflammatory Effects of Mannanase-Hydrolyzed Copra Meal in a Porcine Model of Colitis

doi: 10.1292/jvms.13-0424

Figure Lengend Snippet: Effect of MCM on (A) fecal IgA concentrations and (B) plasma CD4+CD25+ cell populations. Data are mean ± SEM; n=3; “*” indicates significant difference from NEG; “ # ” indicates significant difference from POS; P <0.05.

Article Snippet: This cell suspension (50 μl , 10 7 cells/ μl ) was then incubated with mouse anti-pig CD25 (AbD Serotec, Oxford, U.K.), goat anti-mouse IgG1:FITC (AbD Serotec), R-Phycoerythrin-conjugated anti-pig CD4a (BD Biosciences, San Diego, CA, U.S.A.) and anti-mouse/rat FoxP3 Alexa Fluor (eBioscience, San Diego, CA, U.S.A.) according to the manufacturers’ instructions.

Techniques: Clinical Proteomics

Journal: PLoS Pathogens

Article Title: Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

doi: 10.1371/journal.ppat.1006206

Figure Lengend Snippet: Peripheral blood monocytes were isolated from the blood of the wild type (red), heterozygous (blue), and ΔSRCR5 (green) animals. Following cultivation in the presence of recombinant human CSF1 (rhCSF1) for seven days PMMs were analyzed by FACS. A) Co-staining with CD14-FITC and CD16-PE antibodies recognizing the native structure of the proteins (colored contour plots; red wild type, blue heterozygous, green ΔSRCR5) relative to isotype controls (grey). B) Co-staining with CD169-FITC and CD172a-PE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). C) Co-staining with SWC9 (CD203a)-FITC and CD151-RPE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). D) Staining against the native structure of surface expressed CD163 (colored) relative to an isotype control staining (grey).

Article Snippet: Cells were stained with antibodies targeting either mouse anti pig CD14 (AbD Serotec, MGA1273F, 1:50) and mouse anti pig CD16 (AbD Serotec, MCA2311PE, 1:200), mouse anti pig CD169 (AbD Serotec, MCA2316F, 1:50) and mouse anti pig CD172a (SoutherBiotech, 4525–09, 1:400), mouse anti human CD151 (AbD Serotec, MCA1856PE, 1:50) and mouse anti pig SWC9(CD203a) (AbD Serotec, MCA1973F, 1:50), mouse anti pig CD163 (AbD Serotec, MCA2311PE, 1:50), or mouse IgG1 or an IgG2b negative control (AbD Serotec, MCA928PE,MCA691F, or Sigma, F6397; same concentration as primary Ab).

Techniques: Isolation, Recombinant, Staining, Control

Journal: Infection and Immunity

Article Title: Local Host Response to Chlamydial Urethral Infection in Male Guinea Pigs ▿

doi: 10.1128/IAI.01339-09

Figure Lengend Snippet: Kinetics of the cellular response in the urethra following primary and challenge infections as assessed by flow cytometry. Each data point represents the mean and standard deviation for 5 animals. The number of CD8 cells on day 14 was significantly greater than the number of CD4 cells (P = 0.008). The number of B cells following challenge infection was significantly greater than the numbers of CD4 and CD8 T cells on each of the days following challenge (P, <0.01 to 0.001).

Article Snippet: R-phycoerythrin (RPE)-labeled mouse anti-guinea pig CD4 (CT7), fluorescein isothiocyanate (FITC)-labeled mouse anti-guinea pig CD8 (CT6), mouse anti-guinea pig CD45 (IH-1), mouse anti-B-cell subset (MsGp10), and an allophycocyanin (APC) conjugation kit were purchased from AbD Serotec (Oxford, United Kingdom).

Techniques: Flow Cytometry, Standard Deviation, Infection